مجله سلول و بافت
سال دوازدهم شماره 3 (پاییز 1400)

This study aimed to evaluate the expression levels of TINCR in tumor and adjacent non-tumor tissues of 50 women diagnosed with invasive ductal carcinoma. The association of TINCR expression and the clinical characteristics of patients have also been studied.

Total RNA was isolated from tumor and adjacent non- tumor tissues of breast cancer patients using RNX-Plus. Then, PrimeScriptTM RT reagent was used for converting total RNA to cDNA. TINCR lncRNA levels were quantified by qRT-PCR and results was analyzed with paired sample t test. Moreover, for evaluation the TINCR biomarker potential, ROC curve analysis was used in breast cancer tumor tissues.

Reduced levels of TINCR were obtained in tumor tissues of breast cancer patients compared with adjacent non-tumor tissues (P<0.0001). TINCR expression had negative association with tumor size and lymph node metastasis in breast cancer tumor tissues.

TINCR lncRNA downregulation in tumor tissues of breast cancer patients indicates that its expression level could discriminate the tumor from non-tumor tissues in breast cancer patients. Furthermore, TINCR lncRNA have low levels in breast cancer patients with large tumor size, positive lymph node metastasis and luminal A and B subtypes.

In this experimental study, ovarian follicle development was studied using insulin-like growth factor and mesenchymal stem cells.

In this study, mesenchymal stem cells were extracted from the femur bone marrow of 20 mice and 100 pairs of ovaries of mice were co-cultured with insulin-like growth factor (IGF-1) at different concentrations (0.1, 0.5, 1, 5, 10µg) for seven days. Then ovarian follicles were examined and counted by Hematoxylin-eosin, Mason trichrome and PAS staining. Statistical analysis was performed using SPSS software and paired t-test with P<0.05.

Comparison of ovarian follicles in the control group and the experimental groups in most groups showed a significant difference between the follicles (P<0.05). This difference was more evident in the number of preantral follicles in the experimental groups of 5 and 10 μg / ml IGF-1 in the presence and absence of mesenchymal stem cells than in the control group. The number of follicles in the control group was 1.25±1.03 and 13.75±2.71; 10.75±1.28 at concentrations of 5 and 10 μg, respectively. Theca and granulosa layers did not show a significant difference in the control and treated groups.

This method is effective in the growth and development of adult follicles and also minimizes the rate of cellular apoptosis. Obviously, the use of ovarian tissue culture and subsequent production of quality eggs will solve many infertility and genetic problems in the future.

The aim of this study was the effect of adding different levels of vitamin C (0, 1.5, 3, 4.5 and 6 mg / ml) after freezing in semen diluent on the quality parameters of Najdi goat sperm.

Semen samples were collected twice a week from 4 male goats with an average weight of 50± 5 kg by electroojaculator. The sperm samples after cryopreservation were analyzed for sperm motility and velocity characteristics using computerized sperm analysis system (CASA), percentage of sperm survival and abnormal sperm, membrane health and antioxidant capacity.

The results showed that the levels of 3 and 4.5 mg of vitamin C in diluent of goat sperm containing improved total motility, progressive motility and percentage of sperm survival in comparison with the control group (P<0.05). In sperm membrane health, the level of 3 mg of vitamin C was significantly different from the control and the level of 1.5 mg of vitamin C (P <0.05); But there was no significant difference with levels of 4.5 and 6 mg of vitamin C. The percentage of abnormal sperm was not affected by different levels of vitamin C (P <0.05). Sperm antioxidant capacity at the levels of 4.5 and 6 mg of vitamin C, had the highest value and had a significant difference compared to the control treatment (P <0.05).

According to the results of this study, adding 3 and 4.5 mg levels of vitamin C to Tris diluent Najdi goat sperm improves sperm quality parameters and sperm antioxidant capacity after freezing.

Investigation the effects of thermal stress on the arm regeneration of brittle star Ophiocoma scolopendrina and comparing them with samples regenerated in normal temperature.

In this study, brittle star samples of O. scolopendrina collected from Qeshm Island were anesthetized with 3.5% Mgcl2.6 (H2O) solution. Two arms were cut from each star. The brittle stars were divided into 6 groups: three aquariums for control samples (25 ℃) and three aquariums for thermal treatment samples (32 ℃). Then, at 24, 72 hours, 14 and 21 days post amputation, their arms have been amputated again. After fixing and decalcification, the samples were prepared and examined under a light microscope.

Histological studies of the regeneration process in thermal-treated samples showed that the rate of regeneration in them up to 14 days is faster than the control samples, but from this period onwards the temperature tolerance was beyond their tolerance and led to autotomy and disk destruction of these brittle stars. On the other hand, one of the interesting results of this research was that brittle stars under heat stress reconstructed their miniature arm under the epidermis covering the wound site, which may be a mechanism to protect the delicate and sensitive reconstructed arm.

Due to the increase in metabolic rate, the samples of thermal treatment showed more speed in the repair and regeneration processes, but with the prolongation of the time period, the stars underwent autotomy and perished.

The aim of this study was to prepare freeze-dried silk fibroin nanocomposite containing ion copper loaded silk fibroin nanoparticles to induce angiogenesis.

Here, the silk fibroin nanocomposite scaffold containing ion copper was used to induce angiogenesis in endothelial cells. To prepare the silk fibroin nanoparticles containing ion copper, identified amounts of ion copper were added to the fibroin solution. Then, the nanoparticles were embedded in the freeze-dried silk fibroin scaffold. The size and zeta potential, morphology, and the chemical structure of the nanoparticles were measured by dynamic light scattering (DLS), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR). Moreover, the release of ion copper from the scaffold and the biocompatibility of the scaffold were evaluated by atomic absorption spectroscopy (AAS) and MTT assay.

The obtained results showed that adding different concentrations of ion copper to fibroin nanoparticles increased the size of the nanoparticles from 128 nm to 185, 220, and 282 nm. Moreover, the growth and proliferation of HUVEC cells on nanocomposites was increased (140%) compared to the control.

It should be concluded that copper ion doped SFNPs could appropriately induce the proliferation of endothelial cells which consequently influence the angiogenesis.

In assisted reproductive techniques, the selection of a good quality embryo is an important goal, which requires access to a standard method. Today, there are several methods for evaluating in vitro embryos. The morphological evaluation is based on the cleavage rate, shape and symmetry of the blastomeres in the day 3 and day 5 of development. Also, in the preimplantation genetic diagnosis (PGD) method, embryos are evaluated for genetic diseases, to finally are selected a suitable embryo for transfer to the mother's uterus. Since it is important to achieve a low-risk, and high-precision method for selecting of good quality embryos. Recently, the evaluation of embryonic metabolism using analyzing of the material absorbed and secreted by embryos in culture medium, as a non-invasive assessment, has been proposed, that can predict the embryo growth and development more accurately along with morphological evaluation. Therefore, in this narrative review study, relationship between embryo metabolism and its quality has been discussed as a new approach.